Cloned (Comment) | Organism |
---|---|
stable expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C362S/C482S | site-directed mutagenesis | Homo sapiens |
additional information | construction of FXI with a single catalytic active site, construction of recombinant FXI with the A4 domain replaced by the A4 domain from plasma prekallikrein, i.e. FXI/PKA4, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Phe-Pro-Arg-CH2Cl | irreversibly inhibits both active sites of the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of isolated subunits with each one active site, stoichiometry of FIX and FIXalphabeta binding to FXIa, overview | Homo sapiens | |
0.053 | - |
factor IX | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.4 | - |
pyroGlu-Pro-Arg-4-nitroanilide | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor IX + H2O | Homo sapiens | two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX | activated factor IX + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | FXI activation proceeds through an intermediate with one activated subunit, limited digestion by incubating with FXIIa or thrombin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
native enzyme from plasma and recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity chromatography with elution by benzamidine | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cleavage at Ala145 and Ala180, activity of wild-type and mutant enzymes with wild-type andmutant substrate, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor IX + H2O | two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX | Homo sapiens | activated factor IX + ? | - |
? | |
factor IX + H2O | cleavage at Ala145 and Ala180, activity of wild-type and mutant enzymes with wild-type and mutant substrate, overview | Homo sapiens | activated factor IX + ? | - |
? | |
methyl-sulfonyl-D-cyclo-hexyl-glycyl-glycyl-arginine-p-nitroanilide + H2O | substrate S-299 | Homo sapiens | ? | - |
? | |
pyroGlu-Pro-Arg-4-nitroanilide + H2O | substrate S-2366 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | two forms of activated factor XI are generated during coagulation, and each half of a factor XIa dimer behaves as an independent enzyme with respect to factor IX | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FXIa | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.268 | - |
pyroGlu-Pro-Arg-4-nitroanilide | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.55 | - |
factor IX | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |